Web3 jun. 2016 · Methods Athymic nude mice were injected with MCF10AT1 cells in Matrigel® and fed a diet containing either 0.86 % methionine (control fed, CF), or 0.12 % methionine (MR) for 12 weeks. Web12 apr. 2024 · In contrast to MCF10A, treatment with E2 reduced MCF10AT1 shScramble viability by 10.5 ± 9.1% compared to MCF10AT1 + EtOH (p-value < 0.05; Figure 4G). Similar results were observed between MCF10AT1 sh62169 + E2 and MCF10AT1 sh62170 + E2 compared to their respective EtOH conditions; they were, nevertheless, statistically …
Expression of estrogenicity genes in a lineage cell culture model …
WebOur study revealed that exposed MCF10AT1 cells developed, in a time-dependent manner, an acquired phenotype characterized by an increase in cancerous properties (anchorage independent growth and stem-like phenotype). Co-exposure of MCF10AT1 cells to B[a]P and BPA led to a significantly greater aggressive phenotype compared to B[a]P or BPA … Web13 jan. 2024 · It is also unclear how common genetic defects, such as p53 mutations, modify these stromal-epithelial interactions. To address these knowledge gaps, we leveraged the MCF10 progression series of breast cell lines (MCF10A, MCF10AT1, and MCF10DCIS) to develop a longitudinal, tissue-contextualized model of p53-deficient, pre-malignant breast. setting phone to computer
Promotion of breast cancer by beta-hexachlorocyclohexane in …
Web13 mei 2015 · These cell lines include MCF10AT1 (MCF10AT), a premalignant cell line derived from MCF10A transfected with H-Ras , and a set of oncogenic MCF10CA cell … Web3 jun. 2016 · The MCF10AT1 tumors and mammary fat pads were paraffin embedded and sectioned at the Microscopy and Histology Facility, PSU College of Medicine, Hershey, … WebIn MCF10AT1 cells, the MAPK pathway is rapidly activated (increase in the phospho-p42/p44 MAPK/MAPK ratio) in the presence of the selective GPR30 agonist G1 (66) and this activation is impaired in ... setting personal goals