Hif1a antibody
WebHIF1a/HIF-1 alpha antibody used for IHC-Fr on frozen liver sections of pair- or ethanol-fed C57BL/6J wild-type mice (Figure 1B) and hepatocyte-specific HIF1a-deficint mice /delta HepHIF1a-/- on C57BL/6 background fed … WebI am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research.
Hif1a antibody
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WebDetection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells that were treated with 200 µM cobalt chloride.Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate.Detection: … WebDetection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl 2 was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram).To facilitate intracellular staining, …
WebBethyl Laboratories Anti-HIF1-alpha Polyclonal, Catalog # A300-286A. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC) and … WebA specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal …
Web23 de jul. de 2024 · HIF1a Antibody (28b) is a mouse monoclonal IgG 1 κ HIF1a antibody, cited in 244 publications, provided at 200 µg/ml epitope mapping within amino … WebHIF1A Antibody (PA5-85494) in ICC/IF. Immunofluorescence analysis of Hypoxia-inducible factor 1-alpha was performed using 70% confluent log phase MCF7 cells treated with 150 µM of Cobalt chloride for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with ...
Web21 de mar. de 2024 · HIF1A (Hypoxia Inducible Factor 1 Subunit Alpha) is a Protein Coding gene. Diseases associated with HIF1A include Retinal Ischemia and Enchondromatosis, …
WebUnder normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O 2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia … chippy renjith movies and tv showsWebHIF1a/HIF-1 alpha antibody used for IHC-Fr on frozen liver sections of pair- or ethanol-fed C57BL/6J wild-type mice (Figure 1B) and hepatocyte-specific HIF1a-deficint mice /delta HepHIF1a-/- on C57BL/6 background … grapes of wrath turtleWebHIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。 Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使 … chippy rocket leagueWebAnti-HIF1 alpha Antibody [HIF1A/84] (A248827) 100µg / £335. Description: Mouse monoclonal [HIF1A/84] antibody to HIF1 alpha. Applications: Flow Cytometry, IF: Reactivity: Human: Conjugate: Unconjugated: View Product. Anti-CALR Antibody (A85185) 10µg - 100µg / £105 – £305. Description: Goat polyclonal antibody to CALR. Applications: chippy robot chipotleWebHIF1A Polyclonal Antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. … grapes of wrath turtle crossing the roadWebHIF1α-siRNA and gemcitabine combination-based GE-11 peptide antibody-targeted nanomedicine for enhanced therapeutic efficacy in ... respectively. Overall, present work lays foundation for the combination of GEM and HIF1a-siRNA loaded in a targeted nanocarrier system as a unique therapeutic option in pancreatic cancer treatment ... chippy rhos on seaWebAlternative Name: HIF1; HIF-1A; HIF1A; HIF-1-alpha; HIF1-alpha; BHLHE78; MOP1; PASD8 Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ ... Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. chippy residency chennai